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邱吉国/副教授

发布时间:2022年10月10日 17:14 | 编辑:成丹 | 作者:

邱吉国,博士,副教授,美高梅mgm1888网站微生物系。

研究方向:环境微生物学、微生物生理学等。

招生:硕士研究生导师。欢迎致力于微生物学研究、勤奋努力、具有团队合作精神的学生报考!

邮箱:qiujiguo@njau.edu.cn      电话:025-84396685

General information

Name:      Jiguo Qiu

Affiliation:  Department of Microbiology, College of Life Sciences, Nanjing Agricultural University, Key Laboratory of Agricultural and Environmental Microbiology, Ministry of Agriculture and Rural Affairs, Nanjing 210095, China.

Tel:        (86)-25-84396685

Fax:       (86)-25-84396314

Email:       qiujiguo@njau.edu.cn

Personal schoolar website:

https://www.researchgate.net/profile/Jiguo_Qiu

https://scholar.google.com/citations?hl=zh-CN&user=KTOLjJ0AAAAJ&view_op=list_works&sortby=pubdate


Education and Employment History

  1. 01/2019-present  Associate Prof. in Microbiology, College of Life Sciences, Nanjing Agricultural University, China.

  2. 07/2019-08/2020  Visiting Scholar in Microbiology, School of Medicine, University of Washington, Seattle, USA.

  3. 04/2016-12/2018  Assistant Prof. in Microbiology, College of Life Sciences, Nanjing Agricultural University, China.

  4. 04/2014-04/2016  Postdoc in Environmental Science and Technology, College of Environment, Zhejiang University of Technology, China.

  5. 09/2010-03/2014  Ph.D. in Environmental Science, College of Environmental and Resource Sciences, Zhejiang University, China.

  6. 09/2006-06/2009  M.S. in Microbiology, College of Life Sciences, Nanjing Agricultural University, China.

  7. 09/2001-06/2005  B.S. in Sericulture, School of Forestry, Shandong Agricultural University, China


Research Projects

  1. Principal Investigator. The catabolism of phenazine-1-carboxylic acid by bacterial strain Rhodococcus sp. WH99. National Natural Science Foundation of China (No. 32070092). 01/2021-12/2024

  2. Principal Investigator. The catabolism of cotinine in Nocardioides sp. strain JQ2195. National Natural Science Foundation of China (No. 31870092). 01/2019-12/2022

  3. Principal Investigator. Molecular mechanism study on nicotine metabolism by Pusillimonas sp. T2. National Natural Science Foundation of China (No. 31500082). 01/2016-12/2018

  4. Principal Investigator. Characterization and structure analyse of the tetrahydrofolate (THF)-dependent dicamba demethylase. China Postdoctoral Science Foundation (No. 2016M601826). 042016-04/2018


Research experience

  1. My research has involved in the biodegradation of toxic compounds by bacteria: (1) isolates and characterizes novel degrading strains; (2) determines the degrading pathways of compounds; (3) clones novel degradation genes or enzymes using ‘transposon mutagenesis’ and chromosome walking; (4) clones, overexpresses, and purifies the functional enzymes; (5) constructs recombinant for biocatalysis, bioremediation and synthetic biology.

  2. Methods used: (1) Isolation and purification of DNA, RNA, plasmids from environmental samples. (2) High throughput sequencing (phylogenetic and functional marker genes). (3) Quantitative PCR. (4) Phylogenetic analysis, probe and primer design. (5) Cultivation and selecttion of bacteria and mutants. (6) Gene transfer including  conjugation, transduction, classical transformation and electroporation, monitoring of gene transfer events. (7) Gene knockout and complementation. (8) Multivariate statistics. (9) Laboratory-based microbial model systems. (10) Bioinformatics and genomics. (11) Software on biological analysis, such as bioedit, omiga, mega, DNAman.


Selected Publications (*Corresponding author)

  1. Si-Qiong Xu, Xiao Wang, Lu Xu, Ke-Xin Wang, Yin-Hu Jiang, Fu-Yin Zhang, Qing Hong, Jian He, Shuang-Jiang Liu, Ji-Guo Qiu. The MocR family transcriptional regulator DnfR has multiple binding sites and regulates Dirammox gene transcription in Alcaligenes faecalis JQ135. Environmental Microbiology, 2023, https://doi.org/10.1111/1462-2920.16318.

  2. Yang Mu, Siqiong Xu, Guiping Liu, Minggen Cheng, Weixian Dai, Qing Chen, Xin Yan, Qing Hong, Jian He, Jiandong Jiang*, Jiguo Qiu*. A novel monooxygenase gene dipD in dip gene cluster of Alcaligenes faecalis JQ135 is essential for the initial catabolism of dipicolinic acid. Applied and Environmental Microbiology, 2022, 88 (14): e00360-22.

  3. Siqiong Xu, Xiao Wang, Fuyin Zhang, Yinhu Jiang, Yanting Zhang, Minggen Cheng, Xin Yan, Qing Hong, Jian He, Jiguo Qiu*. PicR as a MarR family transcriptional repressor multiply controls the transcription of picolinic acid degradation gene cluster pic in Alcaligenes faecalis JQ135. Applied and Environmental Microbiology, 2022, 88 (11): e00172-22.

  4. Siqiong Xu, Yinhu Jiang, Fuyin Zhang, Xiao Wang, Kaiyun Zhang, Lingling Zhao, Qing Hong, Jiguo Qiu*, Jian He*. The TetR family repressor HpaR negatively regulates the catabolism of 5-hydroxypicolinic acid in Alcaligenes faecalis JQ135 by binding to two unique DNA sequences in the promoter of hpa operon. Applied and Environmental Microbiology, 2022, 88 (6): e02390-21.

  5. Siqiong Xu, Xinxin Qian, Yinhu Jiang, Yaling Qin, Fuyin Zhang, Kaiyun Zhang, Qing Hong, Jian He, Lili Miao, Zhipei Liu, Defeng Li, Shuangjiang Liu*, Jiguo Qiu*. Genetic foundations of direct ammonia oxidation (Dirammox) to N2 and MocR-like transcriptional regulator DnfR in Alcaligenes faecalis JQ135. Applied and Environmental Microbiology, 2022, 88 (6): e02261-21.

  6. Lingling Zhao, Zhenyang Zhao, Kaiyun Zhang, Xuan Zhang, Siqiong Xu, Junwei Liu, Bin Liu, Qing Hong, Jiguo Qiu*, Jian He. Cotinine hydroxylase CotA initiates biodegradation of wastewater micropollutant cotinine in Nocardioides sp. strain JQ2195. Applied and Environmental Microbiology, 2021, 87 (18): e00923-21.

  7. Jiguo Qiu, Lingling Zhao, Siqiong Xu, Qing Chen, Le Chen, Bin Liu, Qing Hong, Zhenmei Lu, Jian He*. Identification and characterization of a novel pic gene cluster responsible for picolinic acid degradation in Alcaligenes faecalis JQ135. Journal of Bacteriology, 2019, 201 (16): e00077-19.

  8. Jiguo Qiu, Yanting Zhang, Shigang Yao, Hao Ren, Meng Qian, Qing Hong, Zhenmei Lu*, Jian He*. Novel 3, 6-dihydroxypicolinic acid decarboxylase mediated picolinic acid catabolism in Alcaligenes faecalis JQ135. Journal of Bacteriology, 2019, 201 (7): e00665-18.

  9. Jiguo Qiu, Bin Liu, Lingling Zhao, Yanting Zhang, Dan Cheng, Xin Yan, Jiandong Jiang, Qing Hong, Jian He*. A novel degradation mechanism for pyridine derivatives in Alcaligenes faecalis JQ135. Applied and Environmental Microbiology, 2018, 84 (15): e00910-18.

  10. Jiguo Qiu, Yin Wei, Yun Ma, Rongti Wen, Yuezhong Wen, Weiping Liu*. A novel (S)-6-hydroxynicotine oxidase gene from Shinella sp. strain HZN7. Applied and Environmental Microbiology, 2014, 80 (18): 5552-5560.

  11. Jiguo Qiu, Yun Ma, Jing Zhang, Yuezhong Wen, Weiping Liu*. Cloning of a novel nicotine oxidase gene from Pseudomonas sp. strain HZN6 whose product nonenantioselectively degrades nicotine to pseudooxynicotine. Applied and Environmental Microbiology, 2013, 79 (7): 2164-2171.

  12. Jiguo Qiu, Yun Ma, Yuezhong Wen, Liansheng Chen, Lifei Wu, Weiping Liu*. Functional identification of two novel genes from Pseudomonas sp. strain HZN6 involved in the catabolism of nicotine. Applied and Environmental Microbiology, 2012, 78 (7): 2154-2160.



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